http://weekly.chinacdc.cn/ China CDC Weekly cdcwjournal@163.com cdcwjournal@163.com en cdcwjournal@163.com 2096-7071 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.088?pageType=en Roujian Lu, Peihua Niu, Li Zhao, Huijuan Wang, Wenling Wang, Wenjie Tan <sec<<p< <b<What is already known on this topic?</b< </p<</sec<<sec<<p<Coronavirus disease 2019 (COVID-19), a disease caused by a novel human coronavirus named the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or COVID-19 virus, was reported in December 2019. Complete genomes of the COVID-19 virus from clinical samples using next generation sequencing (NGS) have been reported.</p<</sec<<sec<<p< <b<What is added by this report?</b< </p<</sec<<sec<<p<Here we provide the technical data for sequencing complete genome of COVID-19 virus from clinical samples using the Sanger method. Two complete COVID-19 virus genome sequences (named WH19004-S and GX0002) were obtained from clinical samples of COVID-19 patients, and two single nucleotide polymorphisms (SNPs) in ORF7a (T/C, nt 27,493) and ORF8 (T/C, nt 28,253) of WH19004-S were identified by Sanger sequencing.</p<</sec<<sec<<p< <b<What are the implications for public health practice?</b< </p<</sec<<sec<<p<The COVID-19 virus genome sequencing by Sanger method reported here could be used to generate data of high enough quality without requirement for expensive NGS equipment, which support sequencing complete genomes from clinical samples and monitoring of viral genetic variations of COVID-19 infections.</p<</sec< China CDC Weekly. 2020 2(25): 447-452. Roujian Lu, Peihua Niu, Li Zhao, Huijuan Wang, Wenling Wang, Wenjie Tan <sec<<p< <b<What is already known on this topic?</b< </p<</sec<<sec<<p<Coronavirus disease 2019 (COVID-19), a disease caused by a novel human coronavirus named the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) or COVID-19 virus, was reported in December 2019. Complete genomes of the COVID-19 virus from clinical samples using next generation sequencing (NGS) have been reported.</p<</sec<<sec<<p< <b<What is added by this report?</b< </p<</sec<<sec<<p<Here we provide the technical data for sequencing complete genome of COVID-19 virus from clinical samples using the Sanger method. Two complete COVID-19 virus genome sequences (named WH19004-S and GX0002) were obtained from clinical samples of COVID-19 patients, and two single nucleotide polymorphisms (SNPs) in ORF7a (T/C, nt 27,493) and ORF8 (T/C, nt 28,253) of WH19004-S were identified by Sanger sequencing.</p<</sec<<sec<<p< <b<What are the implications for public health practice?</b< </p<</sec<<sec<<p<The COVID-19 virus genome sequencing by Sanger method reported here could be used to generate data of high enough quality without requirement for expensive NGS equipment, which support sequencing complete genomes from clinical samples and monitoring of viral genetic variations of COVID-19 infections.</p<</sec< China CDC Weekly. 2020 2(25): 447-452. Sequencing the Complete Genome of COVID-19 Virus from Clinical Samples Using the Sanger Method Roujian Lu, Peihua Niu, Li Zhao, Huijuan Wang, Wenling Wang, Wenjie Tan 2020-06-01 Personal use only, all commercial or other reuse prohibited China CDC Weekly. 2020 2(25): 447-452. article doi:10.46234/ccdcw2020.088 10.46234/ccdcw2020.088 China CDC Weekly 2 25 2020-06-01 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.088?pageType=en 447 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.116?pageType=en Peihua Niu, Roujian Lu, Li Zhao, Huijuan Wang, Baoying Huang, Fei Ye, Wenling Wang, Wenjie Tan <sec< <b< <b<What is already known on this topic?</b< </b< <p<A novel human coronavirus, known as SARS-CoV-2 or 2019-nCoV, is the causative agent of the coronavirus disease 2019 (COVID-19). We have released the primers and probes of real-time reverse transcription polymerase chain reaction (rRT-PCR) assays for the laboratory detection of COVID-19 infection.</p<</sec<<sec< <b< <b<What is added by this report?</b< </b< <p<Here we provide detailed technical data and evaluate the performance of three novel rRT-PCR assays targeting the ORF1ab, N, and E genes for detection of COVID-19 infection. The application of rRT-PCR assays among four types of specimens (alveolar lavage, sputum, throat swabs, and stool) from patients with COVID-19 indicated that the mean viral loads detected in sputum were higher than other specimens.</p<</sec<<sec< <b< <b<What are the implications for public health practice?</b< </b< <p<These rRT-PCR assays reported here could be used for laboratory diagnosis of COVID-19 infection with high sensitivity, specificity, and applicability. Sputum rather than throat swabs and stool should be a priority for specimen collection for laboratory detection of COVID-19.</p<</sec< China CDC Weekly. 2020 2(25): 453-457. Peihua Niu, Roujian Lu, Li Zhao, Huijuan Wang, Baoying Huang, Fei Ye, Wenling Wang, Wenjie Tan <sec< <b< <b<What is already known on this topic?</b< </b< <p<A novel human coronavirus, known as SARS-CoV-2 or 2019-nCoV, is the causative agent of the coronavirus disease 2019 (COVID-19). We have released the primers and probes of real-time reverse transcription polymerase chain reaction (rRT-PCR) assays for the laboratory detection of COVID-19 infection.</p<</sec<<sec< <b< <b<What is added by this report?</b< </b< <p<Here we provide detailed technical data and evaluate the performance of three novel rRT-PCR assays targeting the ORF1ab, N, and E genes for detection of COVID-19 infection. The application of rRT-PCR assays among four types of specimens (alveolar lavage, sputum, throat swabs, and stool) from patients with COVID-19 indicated that the mean viral loads detected in sputum were higher than other specimens.</p<</sec<<sec< <b< <b<What are the implications for public health practice?</b< </b< <p<These rRT-PCR assays reported here could be used for laboratory diagnosis of COVID-19 infection with high sensitivity, specificity, and applicability. Sputum rather than throat swabs and stool should be a priority for specimen collection for laboratory detection of COVID-19.</p<</sec< China CDC Weekly. 2020 2(25): 453-457. Three Novel Real-Time RT-PCR Assays for Detection of COVID-19 Virus Peihua Niu, Roujian Lu, Li Zhao, Huijuan Wang, Baoying Huang, Fei Ye, Wenling Wang, Wenjie Tan 2020-06-01 Personal use only, all commercial or other reuse prohibited China CDC Weekly. 2020 2(25): 453-457. article doi:10.46234/ccdcw2020.116 10.46234/ccdcw2020.116 China CDC Weekly 2 25 2020-06-01 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.116?pageType=en 453 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.115?pageType=en Cao Chen, Xiang Zhao, Dayan Wang, Juan Li, Ao Wang, Donglin Wu, Zhixiao Chen, Yuchao Wu, Yao Meng, Weifeng Shi, George F. Gao, Guizhen Wu, Biao Huang, Wenbo Xu China CDC Weekly. 2020 2(25): 458-459. Cao Chen, Xiang Zhao, Dayan Wang, Juan Li, Ao Wang, Donglin Wu, Zhixiao Chen, Yuchao Wu, Yao Meng, Weifeng Shi, George F. Gao, Guizhen Wu, Biao Huang, Wenbo Xu China CDC Weekly. 2020 2(25): 458-459. The Initial Case of COVID-19 — Shulan City, Jilin Province, China, May 8, 2020 Cao Chen, Xiang Zhao, Dayan Wang, Juan Li, Ao Wang, Donglin Wu, Zhixiao Chen, Yuchao Wu, Yao Meng, Weifeng Shi, George F. Gao, Guizhen Wu, Biao Huang, Wenbo Xu 2020-06-01 Personal use only, all commercial or other reuse prohibited China CDC Weekly. 2020 2(25): 458-459. article doi:10.46234/ccdcw2020.115 10.46234/ccdcw2020.115 China CDC Weekly 2 25 2020-06-01 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.115?pageType=en 458 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.127?pageType=en Jun Xu, Yong Zhang, Xiang Zhao, Dayan Wang, Weiping Dai, Guangyu Jiao, Shiwen Wang, Jun Liu, Weifeng Shi, Cao Chen, Shuhong Chen, Shichun Yan, Hua Hua, George F. Gao, Wenbo Xu, Guizhen Wu China CDC Weekly. 2020 2(25): 460-462. Jun Xu, Yong Zhang, Xiang Zhao, Dayan Wang, Weiping Dai, Guangyu Jiao, Shiwen Wang, Jun Liu, Weifeng Shi, Cao Chen, Shuhong Chen, Shichun Yan, Hua Hua, George F. Gao, Wenbo Xu, Guizhen Wu China CDC Weekly. 2020 2(25): 460-462. A Reemergent Case of COVID-19 — Harbin City, Heilongjiang Province, China, April 9, 2020 Jun Xu, Yong Zhang, Xiang Zhao, Dayan Wang, Weiping Dai, Guangyu Jiao, Shiwen Wang, Jun Liu, Weifeng Shi, Cao Chen, Shuhong Chen, Shichun Yan, Hua Hua, George F. Gao, Wenbo Xu, Guizhen Wu 2020-06-01 Personal use only, all commercial or other reuse prohibited China CDC Weekly. 2020 2(25): 460-462. article doi:10.46234/ccdcw2020.127 10.46234/ccdcw2020.127 China CDC Weekly 2 25 2020-06-01 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.127?pageType=en 460 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.126?pageType=en Canyou Zhang, George F. Gao, Zhifeng Wang, Hao Wu China CDC Weekly. 2020 2(25): 463-466. Canyou Zhang, George F. Gao, Zhifeng Wang, Hao Wu China CDC Weekly. 2020 2(25): 463-466. Fighting Against COVID-19 at the Community Level — Wuhan City, Hubei Province, China, 2020 Canyou Zhang, George F. Gao, Zhifeng Wang, Hao Wu 2020-06-01 Personal use only, all commercial or other reuse prohibited China CDC Weekly. 2020 2(25): 463-466. article doi:10.46234/ccdcw2020.126 10.46234/ccdcw2020.126 China CDC Weekly 2 25 2020-06-01 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.126?pageType=en 463 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.109?pageType=en China CDC Weekly. 2020 2(25): 467-468. China CDC Weekly. 2020 2(25): 467-468. Reported Cases and Deaths of National Notifiable Infectious Diseases — China, April, 2020 2020-06-01 Personal use only, all commercial or other reuse prohibited China CDC Weekly. 2020 2(25): 467-468. article doi:10.46234/ccdcw2020.109 10.46234/ccdcw2020.109 China CDC Weekly 2 25 2020-06-01 http://weekly.chinacdc.cn/article/doi/10.46234/ccdcw2020.109?pageType=en 467

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